The dye has obviously partitioned toward the aqueous layer, which is consistent with its very polar structure (Figure 4.46). At the CERN particle accelerator in Geneva, Switzerland, protons are accelerated to a speed of 0.999999972c0.999999972 c0.999999972c. In this particular case K saccharin is a large number because saccharin is more soluble in ether than water while K salt is a small number because salt is slightly soluble in ether. NaCl) to regulate the pH and osmolarity of the lysate. \(^5\)When assessing the result of a litmus paper test, look at the center of the drop. The final Sodium Bicarbonate concentration used in the medium depends on the media formulation and the carbon dioxide concentration used in the incubator. For an organic compound, it is relatively safe to assume that it will dissolve better in the organic layer than in most aqueous solutions unless it has been converted to an ionic specie, which makes it more water-soluble. \(^7\)From: Fessenden, Fessenden, Feist, Organic Laboratory Techniques, 3\(^\text{th}\) ed., Brooks-Cole, 2001. Removes water at a moderate rate, so the solution should be allowed to sit with the drying agent for some time. wOYfczfg}> \(^8\)Blue Drierite is expensive, so is commonly used by mixing it together with white Drierite (\(\ce{CaSO_4}\) without the cobalt indicator). However, if carbon dioxide is passed in excess, it forms the soluble calcium hydrogen-carbonate. Sodium bicarbonate is often referred to as a "systemic" antacid because the unreacted fraction is readily . Write structural formula(condensed) for all the primary , secondary and tertiary haloalkanes An alcohol has the molecular formula C4H10O write the structural formulae of the isomers to show See all questions in Quick Introduction of Structures. This strategy saves steps, resources and time, and most of all, greatly reduces waste. Addition of more anhydrous \(\ce{MgSO_4}\) made the drying agent pinker (Figure 4.45b), as more dye was removed from solution. Why is acid alcohol used as a decolorizing agent? Background Extraction is a frequently used technique to selectively transfer a compound of interested from one solvent to another. The bottom layer is always removed first independently if this is the one of interest or not because it is much easier to do. If the aqueous layer is on the top of a separatory funnel, insert a glass stirring rod into the top layer and touch the wet rod to blue litmus paper. Why are hematoxylin and eosin staining used in histopathology? What would have happened if 5% NaOH had been used? removing impurities from compound of interest. Sodium Bicarbonate | NaHCO3 or CHNaO3 | CID 516892 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological . To demonstrate the effectiveness of a water wash, a Fischer esterification reaction was conducted to produce isoamyl acetate (Figure 4.38). Why does bicarbonate soda and vinegar react? As a base, its primary function is deprotonation of acidic hydrogen. Additionally, ionic solutions have high dielectric constants, making them less compatible with organic compounds. Process of removing a compound of interest from a solution or solid mixture. to the solubility.Extraction becomes a very useful tool if you choose a suitable extraction solvent. Figure 4.47a shows addition of one drop of red food dye to a layer of water in a separatory funnel, and the dye dissolves easily even without swirling. %PDF-1.3 Why does a volcano erupt with baking soda and vinegar? 75% (4 ratings) for this solution. The salt water works to pull the water from the organic layer to the water layer. After separation of the organic and the aqueous layer, the amine can be recovered by addition of a strong base like NaOH or KOH to the acidic extract i.e., lidocaine synthesis. Charged species are soluble in water and other polar solvents, but nonpolar compounds are not. Introduction Extraction is a widely used method for the separation of a substance from a mixture. Quickly removes water well, although larger quantities are needed than other drying agents (holds \(0.30 \: \text{g}\) water per \(\text{g}\) desiccant). We are not going to do that in order to decrease the complexity of the method. For example, it is safely used in the food and medical industry for various applications. The purpose of this wash is to remove large amounts of water than may be dissolved in the organic layer. A solution is nearing dryness when fine particles are noticed that don't cling to other particles (Figure 4.52a+c) or to the glass when swirled (Figure 4.53a). The work-up refers to methods aimed at purifying the material, and most commonly occur in a separatory funnel. In order to effect the extraction, the two solvents must be immiscible, which means that neither dissolves in the other. << /Length 5 0 R /Filter /FlateDecode >> This difference in acidity can be exploited to separate carboxylic acids and phenols from each other in an organic layer. The most useful drying agents indicate when they have completely absorbed all of the water from the solution. Figure 3 shows the mechanism for the synthesis of tert-Butyl chloride from tert-Butyl alcohol using hydrochloric acid. Question 1. Why does sodium chloride dissolve in water? Below are several problems that have been frequently encountered by students in the lab: 6. With a finger placed atop a glass pipette, insert the pipette into the separatory funnel so the tip is positioned in the bottom aqueous layer (Figure 4.42a). Ketones and aldehydes undergo condensation reactions catalyzed by both, acids and bases. 4 In the hospital, aggressive fluid resuscitation with . How much solvent/solution is used for the extraction? Why is smoke produced when propene is burned? x)#fa jvsACREy4OyEf#4mo4u0t:_k}h)pgai^m|~9?/yowm~_7yxwg/W50tU_5Np This page titled 4.7: Reaction Work-Ups is shared under a CC BY-NC-ND 4.0 license and was authored, remixed, and/or curated by Lisa Nichols via source content that was edited to the style and standards of the LibreTexts platform; a detailed edit history is available upon request. Which sequence is the most efficient highly depends on the target molecule. If using \(\ce{MgSO_4}\), gravity filter the solution into an appropriately sized round-bottomed flask (Figure 4.53c). If a large amount of a compound has to be transferred or neutralized, more concentrated solutions and larger quantities might be needed. Use ACS format. From this point of view, a solvent with higher density than water would be preferential, especially when very small quantities are used. Strictly speaking, the two operations are targeting different parts in the mixture: while the extraction removes the target compound from an impure matrix, the washing removes impurities from the target compound i.e., water by extraction with saturated sodium chloride solution. Bicarbonate ion has the formula {eq}HCO_3^-{/eq}. The formation of CO 2 results in belching and gastric distention. . This means that solutions of carbonate ion also often bubble during neutralizations. 4 0 obj Solid/Liquid - teabag in hot water. Many of these neutral compounds tend to react in undesired ways i.e., esters undergo hydrolysis upon contact with strong bases or strong acids. A similar observation will be made if a low boiling solvent is used for extraction. saturated \(\ce{NaHCO_3}\), \(\ce{NaCl}\), or \(\ce{NH_4Cl}\)). A drawback to using \(\ce{MgSO_4}\) is that it is a fine powder, and so the solutions must be subsequently filtered to remove the drying agent. Summary. This is the weird part. Solid can slow drainage in the filter paper. Any ECG signs of hyperkalemia warrant treatment with calcium chloride, beta agonist (albuterol), insulin/glucose and sodium bicarbonate. don't want), we perform an "extraction". << /Length 5 0 R /Filter /FlateDecode >> If NaHCO 3 is used for extraction, the centrifuge tube has to be vented more frequently. The reason sodium carbonate is added to the tea is because the tannins are acidic and sodium carbonate is a base, so when sodium carbonate is added to the tea water mixture, the acids are . The purpose of washing the organic layer with saturated sodium chloride is to remove the . Practical Aspects of an Extraction This can be use as a separation First, add to the mixture NaHCO3. a. Draw the reaction between acetylsalicylic acid and NaHCO 3 then draw the reaction between that product and HCl. However, they do react with a strong base like NaOH. What is the purpose of using washing buffer during RNA extraction? Why is bicarbonate the most important buffer? Why is sulphur dioxide used by winemakers? In this context it would be wise to label all layers properly in order to be able to identify them correctly later if necessary. Many organic solvents dissolve a significant portion of water (Table 4.6) that must be removed before rotary evaporation, or else water will be found in the concentrated product. Why use sodium bicarbonate in cardiac arrest? have a stronger attraction to water than to organic solvents. Why is eriochrome black T used in complexometric titration? Touch the aliquot to blue litmus paper and observe the color (Figure 4.42d). O A Because NaHCO3 deprotonates 3-chlorobenzoic acid and NaOH does not O B Because NaOH deprotonates both 3-chlorobenzoic acid and 2-naphthol 0 C Because NaHCO3 deprotonates both 3-chlorobenzoic acid and This problem has been solved! Never dispose of any layer away until you are absolutely sure (=100 %) that you will never need it again. The sodium bicarbonate wash in this example was necessary (and discussed in the next section) because a water wash alone may not fully remove the acetic acid. Bicarbonate ion has the formula HCO 3 H C O. 5. - prepare 2 m.p. Depending on the chain length, amines might or might not be soluble in water i.e., propylamine is miscible with water (log Kow=0.48), triethylamine displays a limited solubility at room temperature (17 g/100 mL, log Kow=1.44), while tributylamine hardly dissolves at all (0.37 g/100 mL, log Kow=4.60). Explore the definition and process of solvent extraction and discover a sample problem. How can I draw the following amines: butan-1-amine, pentan-2-amine, propan-1,2-diamine? If the entire drying agent clumps into pieces that are much larger than the original size (Figure 4.52b+c), there is still water remaining in the flask. At 2 h after CPR, the brain, heart, and lung were collected and mRNA extraction, followed by cDNA synthesis and real-time PCR were performed. b. Why is baking soda and vinegar endothermic? 4 0 obj If drying agents are used to remove water, you might wonder "Why bother with brine; why not use lots of drying agent when the time comes?" In order to separate compounds from each other, they are often chemically modified to make them more ionic i.e., convert a carboxylic acid into a carboxylate by adding a base. In addition, the stopper on the top has to fit into the joint on the top to prevent leakage there (for more details at the end of this chapter). samples of the OG mixture to use later. To demonstrate, Figure 4.45 shows an ethyl acetate solution that has a faint pink tint because it contains some dissolved red food dye. When the goal of an experiment is to conduct a reaction and isolate the product, the general sequence of events is shown in Table 4.4. The organic material in the liquid decays, resulting in increased levels of odor. We receieved your request, Stay Tuned as we are going to contact you within 1 Hour. Ca (OH)2 + CO2 CaCO3 + H2O Its high surface area means it will somewhat adsorb compound: be sure to rinse after filtering. Hence, the solution becomes basic due to the presence of additional OH- ions produced by hydrolysis. The 2-naphthol was extracted from the organic layer by adding 20mL of cold 10% aqueous sodium hydroxide solution to the 125mL separatory funnel. The three most common types of extractions are: liquid/liquid, liquid/solid , and acid/base (also known as a chemically active extraction). Oxygen containing solvents are usually more soluble in water (and vice versa) because of their ability to act as hydrogen bond donor and hydrogen bond acceptor. As expected, a significant signal for acetic acid is seen at \(2.097 \: \text{ppm}\). Plz answer me. Identify one cation and one anion in the given unknown salt m1 by performing dry tests. alcohols, carboxylic acids) can hydrogen-bond with water and increase the likelihood of water dissolving in the organic layer. Early C. elegans embryos display mosaic determination, whereas early mouse embryos exhibit regulative determination. In this reaction, an excess of acetic acid is used to drive the reaction through Le Chatelier's principle, and the acetic acid had to be removed from the product during the purification process. All of these solutions help to modify the (organic) compound and make it more water-soluble and therefore remove it from the organic layer. It's important to know that when a compound is "water soluble" it does not necessarily mean it is "organic insoluble", a common misconception that arises from the "like dissolves like" principle. Another drawback to \(\ce{MgSO_4}\) is that all fine powders heavily adsorb product on their surface (which is why they must be rinsed with solvent after filtration), and sometimes more granular drying agents are used to minimize the loss of product by adsorption. 59 Experimental Procedure 1) Mix isopentyl alcohol (5.4 mL, via burette) and glacial acetic acid (8.5 mL, via graduated . After the reaction is complete, you will remove the excess acetic acid and sulfuric acid from the reaction mixture by extraction with sodium hydrogen carbonate. In many situations drying agents are interchangeable (see Table 4.8 for a survey of drying agents). Why is bicarbonate low in diabetic ketoacidosis? The bubbling was even more vigorous when the layers were mixed together. Hey there! around the world. Which layer is the aqueous layer? Why was 5% sodium bicarbonate used in extraction? Calculate the concentration of H+\mathrm{H}^{+}H+ions in a 0.62M0.62 M0.62M NaOH\mathrm{NaOH}NaOH solution. Why might a chemist add a buffer to a solution? E>!E?h!I'Xyg6WqfB%t]` B;h_~m3wRq`ja4AM$@0?x/,P:a=93X=v4{g5:YAp[Lkcb-kPl`b. A strong base such as sodium hydroxide is not necessary in this particular case. If NaHCO3 is used for extraction, the centrifuge tube has to be vented more frequently. After the extraction, the phenol can be recovered by adding a mineral acid to the basic extract. Why potassium is more reactive than sodium. The presence of water with the product makes the yield inaccurate, and water also must be removed before GC-MS analysis, as water is incompatible with mass-spectrometer detectors. In order to separate these compounds from each other, chromatographic techniques are often used, where the compounds are separated based on their different polarities (see Chromatography chapter). Step 2) DCM extraction NOTE: Chromic s method separates the water first to increase the yield. western blot for protein, or for DNA extraction).Most lysis buffers contain buffering salts (e.g. The shaking of the mixture increases the surface area, and therefore the apparent vapor pressure of the solvent. because CO2 is released during the procedure. All rights reserved. ), sodium bicarbonate should be used. Why should KMnO4 be added slowly in a titration? NaHco3 allows us to just ionize the acid; any base would deprotonate the stronger acid, it's more about not also deprotonating the phenol. Figure 4.47d shows the brine layer containing the dye after shaking with a portion of ethyl acetate. Get access to this video and our entire Q&A library. Note that amides are usually not basic enough to undergo the same protonation (pKa of conjugate acid: ~ -0.5). If 5% sodium hydroxide had been used the ester will be hydrolyzed, yielding a carboxylate salt and an alcohol as products. Why use methyl orange instead of phenolphthalein as a pH indicator. With all drying agents, rinse the drying agent (in the flask and in the filter funnel) with a few \(\text{mL}\) of fresh organic solvent, and add the rinsing to the round-bottomed flask (Figure 4.53d). Why is the removal of air bubbles necessary before starting titration? Sodium bicarbonate is a salt that breaks down to form sodium and bicarbonate in water. This breakdown makes a solution alkaline, meaning it is able to neutralize acid. The \(\ce{^1H}\) NMR spectrum in Figure 4.39a was taken of the reaction mixture immediately after ceasing heating and before the work-up. The reaction affords carbon dioxide (CO2), which is a gas at ambient temperature. Step-by-step solution. Give the purpose of washing the organic layer with saturated sodium chloride. Ethanol, methanol, tetrahydrofuran (THF) and acetone are usually not suitable for extraction because they are completely miscible with most aqueous solutions. In such an event, the mixture can be stirred slowly with a glass rod to bring the small droplets together a little faster, which ultimately leads to the formation of a new layer. \" When the lighting light ratio, the absorbance is only related to the concentration.Why is the sodium extraction solution absorbing 10ml . hydroxide base does not produce CO2 like the bicarbonate base does; no CO2 is being generated here when the hydroxide base is added to the ether soln. R. W. et al. b. For instance, epoxides hydrolyze to form diols catalyzed by acids and bases. This undesirable reaction is called saponification. For instance, if the target compound was the base in the system, the extraction with HCl should be performed first. Since most of the extractions are performed using aqueous solutions (i.e., 5 % NaOH, 5 % HCl), the miscibility of the solvent with water is a crucial point as well as the compatibility of the reagent with the compounds and the solvent of the solution to be extracted. Why is EDTA used in complexometric titration? The organic layer now contains basic alkaloids, while the aq. \(^4\)A. Seidell, Solubilities of Inorganic and Organic Substances, D. Van Nostrand Company, 1907. the solution was swirled with white anhydrous \(\ce{MgSO_4}\), and the drying agent turned pink as it adsorbed the red food dye compound (Figure 4.45a). Your paramedic crew responds to a cardiac arrest in a large shopping complex. Safety note: To prevent excess pressure form being generated by the release of carbon dioxide gas into a separatory funnel during neutralization, the layers should be gently swirled together before placement of the stopper. The higher water solubility lowers the solubility of weakly polar or non-polar compounds in these solvents i.e., wet Jacobsen ligand in ethyl acetate. Ammonium salts from primary amines are much more soluble in water than salts from tertiary amines due the increased ability to form hydrogen bonds [(H3NEt)Cl: 280 g/100 g H2O, (H2NEt2)Cl: 232 g/100 g H2O, (HNEt3)Cl: 137 g/100 g H2O (all at 25 oC)]. A. R You can use extraction to separate a substance selectively from a mixture, or to remove unwanted impurities from a solution.In the practical use, usually one phase is a water or water - based (aqueous) solution and the other an organic One has to keep this in mind as well when other compounds are removed. Explanation: You have performed the condensation. A bit of liquid should remain in the pipette tip, an aliquot of the bottom layer (Figure 4.42c). Water can potentially remove water-soluble impurities from an organic layer, as long as they are present in quantities that do not exceed their water solubility. It is formed from the neutralization of a strong base, namely Sodium hydroxide (NaOH), and . Thus, the density of a solid i.e., sodium hydroxide (2.1 g/cm3 in the solid) does not provide the information sought. 1. add 10-15 mL of 0.5 M NaHCO3 to the ether solution; shake funnel & allow layers to separate. The initial product of reaction (1) is carbonic acid \(\left( \ce{H_2CO_3} \right)\), which is in equilibrium with water and carbon dioxide gas. Extraction involves dissolving a compound or compounds either (1) from a solid into a solvent or (2) . It reacts almost instantaneously to neutralize HCl to produce CO 2 and NaCl. Saturated ionic solutions may be used to decrease the solubility of organic compounds in the aqueous layer, allowing more of a compound to dissolve in the organic layer. Extraction with sodium carbonate solution will remove ANY unreacted acid from the organic phase #RC(=O)OH(aq) + Na_2CO_3(aq) rarrunderbrace(RC(=O)O^(-)""^(+)Na)_"water soluble"+Na^(+)""^(-)HCO_3#, 3394 views Why does sodium bicarbonate raise blood pH? A commonly used method of separating a mixture of organic compounds is known as liquid-liquid extraction. In the aqeuous phase, I do not understand where the HCl comes from, shouldn't it be H2CO3 instead? If using pellets, the solution should be allowed to sit for a few minutes, then decanted. At the same time, find out why sodium bicarbonate is used in cooking and baking. In addition, the salt could be used to neutralize your organic layer. In fact, some of the dye precipitated in the funnel (Figure 4.47d) as it had such low solubility in both brine and ethyl acetate. What is the structure of the functional group and the condensed formula for 4,4,5-triethyl What reactants combine to form 3-chlorooctane? In this example, even after filter and rinsing the drying agent with additional solvent, the drying agent remained pink (Figure 4.45c). 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Why is extraction important in organic chemistry? Drying agents are anhydrous inorganic materials that favorably form "hydrates", which incorporate water molecules into their solid lattice structure (for example, \(\ce{Na_2SO_4} \cdot 7 \ce{H_2O}\)). The following are common materials that can be removed with a water wash: unconsumed acid or base, many ionic salts, and compounds that can hydrogen bond with water (have an oxygen or nitrogen atom) and are relatively small (e.g. If the target compound was an acid, the extraction with NaOH should be performed first. Most solutions are relatively diluted (~5 %) and their density is not much different from that of water (i.e., 5 % HCl: 1.02 g/cm3, 5 % NaOH: 1.055 g/cm3). Product Use. Extraction is a fundamental technique used to isolate one compound from a mixture. the gross of the water from the organic layer. 1. The resulting salts dissolve in water. Why is an acidic medium required in a redox titration? Instead, gently rocking the separatory funnel back and forth for 2-3 minutes will accomplish sufficient degree of mixing while minimizing the formation of emulsions. The bulk of the water can often be removed by, shaking or "washing" the organic layer with saturated aqueous sodium chloride (otherwise.